Encapsulation of S. cerevisiae in Poly(glycerol) Silicate Derived Matrices: Effect of Matrix Additives and Cell Metabolic Phase on Long-Term Viability and Rate of Gene Expression
作者： Jason C. Harper , DeAnna M. Lopez , Elizabeth C. Larkin , Megan K. Economides , Sarah K. McIntyre
DOI: 10.1021/CM103525U
关键词:
摘要: Bioencapsulation of living cells into silica materials derived from the sol–gel process has resulted in novel hybrid with exciting functionalities. Despite many successes this field, long-term viability and activity encapsulated remain a significant obstacle to producing practical robust devices, e.g., whole-cell-based biosensors. We report first study on effects various media additives metabolic phase rate inducible gene expression. Saccharomyces cerevisiae (S. cerevisiae) cells, genetically engineered produce yellow fluorescent protein (YFP) response galactose, were poly(glycerol) silicate matrices. Surprisingly, we find that addition components glycerol-silica matrix adversely impacted all cases studied, 1.3, 1.4, or 5.4 fold decrease after only 9 days storage matrices containing yeast pepton...
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sci-hub.se 参考文章(48)
Maria L. Ferrer, Zaira Y. Garcia-Carvajal, Luis Yuste, Fernando Rojo, Francisco del Monte, Bacteria Viability in Sol−Gel Materials Revisited: Cryo-SEM as a Suitable Tool To Study the Structural Integrity of Encapsulated Bacteria Chemistry of Materials. ,vol. 18, pp. 1458- 1463 ,(2006) , 10.1021/CM0522275
Nadine Nassif, Cécile Roux, Thibaud Coradin, Marie-Noëlle Rager, Odile M. M. Bouvet, Jacques Livage, A sol–gel matrix to preserve the viability of encapsulated bacteria Journal of Materials Chemistry. ,vol. 13, pp. 203- 208 ,(2003) , 10.1039/B210167J
D. Granot, M. Snyder, Glucose induces cAMP-independent growth-related changes in stationary-phase cells of Saccharomyces cerevisiae. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 88, pp. 5724- 5728 ,(1991) , 10.1073/PNAS.88.13.5724
T. G. Khonina, O. N. Chupakhin, L. P. Larionov, T. G. Boyakovskaya, A. L. Suvorov, E. V. Shadrina, Synthesis, toxicity, and percutaneous activity of silicon glycerolates and related hydrogels Pharmaceutical Chemistry Journal. ,vol. 42, pp. 609- 613 ,(2008) , 10.1007/S11094-009-0199-X
Darren R. Dunphy, Todd M. Alam, Michael P. Tate, Hugh W. Hillhouse, Bernd Smarsly, Andrew D. Collord, Eric Carnes, Helen K. Baca, Ralf Köhn, Michael Sprung, Jin Wang, C. Jeffrey Brinker, Characterization of Lipid-Templated Silica and Hybrid Thin Film Mesophases by Grazing Incidence Small-Angle X-ray Scattering Langmuir. ,vol. 25, pp. 9500- 9509 ,(2009) , 10.1021/LA900748R
Molly M Stevens, Julian H George, Exploring and engineering the cell surface interface. Science. ,vol. 310, pp. 1135- 1138 ,(2005) , 10.1126/SCIENCE.1106587
Giovanna Carturan, Roberto Dal Toso, Sara Boninsegna, Renzo Dal Monte, Encapsulation of functional cells by sol–gel silica: actual progress and perspectives for cell therapy J. Mater. Chem.. ,vol. 14, pp. 2087- 2098 ,(2004) , 10.1039/B401450B
Iqbal Gill, Antonio Ballesteros, Encapsulation of Biologicals within Silicate, Siloxane, and Hybrid Sol−Gel Polymers: An Efficient and Generic Approach Journal of the American Chemical Society. ,vol. 120, pp. 8587- 8598 ,(1998) , 10.1021/JA9814568
Bakul C. Dave, Bruce Dunn, Joan Selverstone Valentine, Jeffrey I. Zink, Sol-gel encapsulation methods for biosensors Analytical Chemistry. ,vol. 66, ,(1994) , 10.1021/AC00094A001
T Kemmitt, NB Milestone, The Ring Size Influence on 29Si N.M.R. Chemical Shifts of Some Spirocyclic Tetra- and Penta-coordinate Diolato Silicates Australian Journal of Chemistry. ,vol. 48, pp. 93- 102 ,(1995) , 10.1071/CH9950093