Mg/KCl-ATPase of plant plasma membranes is an electrogenic pump.
作者： H. Sze , K. A. Churchill
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摘要: Abstract The function of the Mg2+-requiring KCl-stimulated ATPase (ATP phosphohydrolase, EC 3.6.1.3) higher plants in active ion transport was investigated by using a purified microsomal fraction containing sealed plasma membrane vesicles. (Sze, H. (1980) Proc. Natl. Acad. Sci. USA 77, 5904-5908). A transmembrane electrical potential (+30 to +44 mV), monitored uptake permeant anion (35SCN-), generated specifically ATP vesicles tobacco callus. ATP-dependent 35SCN- required Mg2+, optimal at pH 6.75, and showed similar concentration dependence as activity. Plasma inhibitors (N,N′-dicyclohexylcarbodiimide vanadate) inhibited generation potential. proton conductor (carbonyl cyanide m-chlorophenylhydrazone), but not K+ ionophore (valinomycin), completely collapsed The results provide vitro evidence that Mg2+/KCl-ATPase is an electrogenic pump. These are consistent with hypothesis H+ pump catalyzed plants.
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