MicroRNA profile indicates downregulation of the TGFβ pathway in sporadic non-functioning pituitary adenomas

摘要: MicroRNAs (miRs) are small, 16–29 nucleotide long, non-coding RNA molecules which regulate the stability or translational efficiency of targeted mRNAs via interference. MiRs participate in control cell proliferation, differentiation, signal transduction, death, and they play a role carcinogenesis. The aims our study were to analyse expression profile miRs sporadic clinically non-functioning pituitary adenomas (NFPA) normal tissues, identify biological pathways altered these tumors. MiR profiles 12 tissue specimens (8 NFPA 4 tissues) determined using miR array based on quantitative real-time PCR with 678 different primers. Five overexpressed mRNA Smads (Smad1-9), MEG DLK1 genes evaluated individual Taqman assays 10 tissues. Pathway analysis was performed by DIANA-mirPath tool. Complex bioinformatical multiple algorithms association studies between miRs, Smad3 tumor size performed. Of 457 expressed both 162 significantly under- compared tissues Expression Smad3, Smad6, Smad9, lower than together silico target prediction indicated possible downregulation TGFβ signaling pathway specific subset miRs. predicted (miR-135a, miR-140-5p, miR-582-3p, miR-582-5p miR-938) overexpressed. Correlation observed seven size. Downregulation through may have complex regulation involved tumorigenesis process NFPA.