Localization and quantitation of expression of two glutamate decarboxylase genes in pancreatic beta-cells and other peripheral tissues of mouse and rat
作者： B E Faulkner-Jones , D S Cram , J Kun , L C Harrison
DOI: 10.1210/ENDO.133.6.8243324
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摘要: Glutamic acid decarboxylase (GAD) catalyzes synthesis of the inhibitory neurotransmitter gamma-amino butyric acid. Two homologous forms GAD encoded by separate genes have been cloned from rat brain, with predicted protein sizes 67 and 65 kilodaltons. is present outside pancreatic islet believed to be a target autoimmunity in insulin-dependent diabetes mellitus. However, peripheral expression two incompletely characterized. We, therefore, investigated tissues, including pancreas, mouse rat. cDNAs encoding were brain shown 95% sequences. RNase protection assay using specific cRNA probes demonstrated both freshly harvested pancreas testis. Levels mRNAs greater than pancreas. mRNA was more abundant 65, localized beta-cells situ hybridization. In t...
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