Prolonged stochastic single ion channel recordings in S-layer protein stabilized lipid bilayer membranes.

摘要: Abstract S-layer proteins are commonly found in bacteria and archaea as two-dimensional monomolecular crystalline arrays the outermost cell membrane component. These have unique property that following disruption by chemical agents, monomers of protein can re-assemble to their original lattice structure. This makes S-layers interesting for utilization bio-nanotechnological applications. Here, we show addition bilayer lipid membranes increases lifetime stability bilayer. M2δ ion channels were functionally incorporated into these stabilized able record activity up 20 h. Transmission electron microscopy (TEM) was used visualize 2D pattern channel characteristics membrane's compared presence absence