Micro-computed tomography for studies on Entobia: transparent substrate versus modern technology

摘要: Endolithic bioerosion is difficult to analyse and describe, it usually requires damaging of the sample material. Sponge erosion (Entobia) may be one most evaluate, as simultaneously macroscopically inhomogeneous microstructurally intricate. We studied traces two Australian sponges Cliona celataand orientaliswith modern technology: high resolution X-ray micro-computed tomography. Micro-CT allows non-destructive visualisation live dead structures in three dimensions was compared traditional microscopic methods. microscopy showed that C. celatabioerosion more intense centre branched out periphery (21 vs. 9% substrate removed). In contrast, orientalisproduced a dense, even meshwork caused an overall pattern than celata(48 central 42% marginal Extended pioneering filaments were not found at margins sponge erosion, but branches ended abruptly or tapered points. Results obtained with micro-CT similar quality observations from transparent optical spar under dissecting microscope. Microstructures could resolved well anticipated. Even though scars chips easily recognisable on maximum magnification images, they lacked detail available SEM. Other drawbacks involve costs presently limited access. cannot replace techniques such epoxy resin casts viewed by SEM, we valuable information. Especially for possibility measure endolithic pore volumes, regard very promising tool will continue optimised. A combination different methods produce best results study Entobia