Telomeric function of mammalian telomerases at short telomeres

摘要: Telomerase synthesizes telomeric sequences and is minimally composed of a reverse transcriptase (RT) known as TERT an RNA TR. We reconstituted heterologous mouse (m) human (h) TERT-TR complexes chimeric mTERT-hTERT-hTR in vitro immortalized alternative lengthening telomere (ALT) cells. Our data suggest that species-specific determinants activity, processivity function map not only to the TR but also component. The presence hTERT-hTR, or complexes, significantly reduced percentage chromosomes without signals ALT Moreover, were defective recruitment telomeres. results requirement for several hTERT domains interaction with multiple proteins proper telomerase shortest telomeres Late-passage mTERT(-/-) embryonic stem (ES) cells ectopically expressing mTERT harboured fewer chromosome ends end-to-end fusions than typically observed late-passage ES ability at inability mechanisms regulating activity might be less stringent