Inhibition of intracellular cAMP-dependent protein kinase using mutant genes of the regulatory type I subunit.
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DOI: 10.1016/S0021-9258(18)45175-7
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摘要: Expression vectors were constructed that code for mutated forms of the regulatory type 1 subunit (RI) cyclic AMP-dependent protein kinase. These mutations alter a specific amino acid which is present in each two homologous cAMP-binding domains RI protein. When these expression introduced into NIH 3T3 and Y1 adrenocortical tumor cells mutant was produced acted dominant fashion to cause 20-400-fold inhibition cAMP-dependent kinase activation. In addition, processes controlled by cAMP adrenal blocked; became resistant growth-inhibitory effects defective steroid synthesis. genes provides means explore role phosphorylation process intracellular signalling.
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