Prevention of acute and chronic allograft rejection by combinations of tolerogenic dendritic cells

摘要: It is well known that adoptive transfer of donor-derived tolerogenic dendritic cells (DC) helps to reduce acute allograft rejection. However, this method cannot effectively prevent grafts from infiltration inflammatory and fibrosis, thus has minimal effect on chronic In study, we used mitomycin C (MMC) generate DC demonstrated donor (Balb/c)-derived MMC-DC could induce hyporesponsiveness recipient (C57BL/6) T in vitro, potentially by inducing T-cell apoptosis, decreasing IL-2 IL-12 secretion, increasing regulatory numbers IL-10 secretion. Furthermore, anti-CD154 monoclonal antibody (mAb) treatment combined with prolonged the survival allografts vivo. The mechanisms were similar those vitro. Impressively, both rejection prevented when F1 generation (Balb/c × C57BL/6) derived injected together mAb into recipients before heart allotransplantation. summary, showed F1-derived have a synergistic induction maintenance regulation secretion immunosuppressive cytokines. Moreover, these two types inhibit transplant mice.