Ret finger protein-like 3 promotes tumor cell growth by activating telomerase reverse transcriptase expression in human lung cancer cells

摘要: // Wangbing Chen 1, 4, * , Jianjun Lu 3, Yu Qin 2, Jingshu Wang Yun Tian 1 Dingbo Shi Shusen Yao Xiao 2 Meng Dai Liu Guo Wei Taihua Wu Bilian Jin Xiangsheng Tie-Bang Kang Wenlin Huang 5 Wuguo Deng Sun Yat-sen University Cancer Center, State Key Laboratory of Oncology in South China, Collaborative Innovation Center Medicine, Guangzhou, China Institute Stem Cell & The First Affiliated Hospital, Dalian Medical University, Dalian, 3 Department Thoracic Surgery, 4 Union Tongji College, Huazhong Science and Technology, Wuhan, Targeted Drug for Tumors Guangdong Province, Guangzhou Double Bioproduct Inc., These authors contributed equally to this article Correspondence to: Wang, e-mail: wangshs@sysucc.org.cn Jin, bjinmcg@gmail.com Deng, dengwg@sysucc.org.cn Received: August 10, 2014      Accepted: October 01, Published: November 13, 2014 ABSTRACT In study, we identified ret finger protein-like (RFPL3) as a hTERT promoter binding protein lung cancer cells. high promoter-binding activity RFPL3 was detected cells compared normal Chromatin immunoprecipitation confirmed tumor-specific protein. Overexpression activated up-regulated expression telomerase activity. Inhibition by siRNA suppressed activation or shRNA also significantly inhibited tumor cell growth vitro xenograft mouse model vivo . Immunohistochemical analysis 181 human adenocarcinomas specimens showed significant correlation between expression. overexpression associated with lymph node metastasis. Univariate multivariate Cox analyses NSCLC clinical revealed strong overall survival. results demonstrate that is an important cellular factor which promotes activating may be potential novel therapeutic target cancer.