Molecular interactions between Hel2 and RNA supporting ribosome-associated quality control
作者： Marie-Luise Winz , Lauri Peil , Tomasz W. Turowski , Juri Rappsilber , David Tollervey
DOI: 10.1038/S41467-019-08382-Z
关键词:
摘要: Ribosome-associated quality control (RQC) pathways monitor and respond to ribosome stalling. Using in vivo UV-crosslinking mass spectrometry, we identified a C-terminal region Hel2/Rqt1 as an RNA binding domain. Complementary crosslinking sequencing data for Hel2 revealed 18S rRNA translated mRNAs. preferentially bound mRNAs upstream downstream of the stop codon. truncation abolished major crosslink polysome association, altered mRNA binding. HEL2 deletion caused loss RQC and, report here, no-go decay (NGD), with comparable effects including RNA-binding site. Asc1 acts asc1∆ impaired mRNA. In conclusion: is recruited or stabilized on translating 40S ribosomal subunits by interactions Asc1. This interaction required function NGD. probably interacts during translation termination.
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