Recombination between paralogues at the Rp1 rust resistance locus in maize.

摘要: Rp1 is a complex rust resistance locus of maize. The HRp1-D haplotype composed Rp1-D and eight paralogues, seven which also code for predicted nucleotide binding site-leucine rich repeat (NBS-LRR) proteins similar to the gene. paralogues are polymorphic (DNA identities 91-97%), especially in C-terminal LRR domain. remaining family member encodes truncated protein that has no Seven nine members, including gene, transcribed. Sequence comparisons between provide evidence past recombination events diversifying selection, particularly half Variants selected complete or partial loss can be explained by unequal crossing over occurred mostly within coding regions. gene altered lost all variants, breakpoints occur throughout genes, most recombinant (9/14 examined) involved same untranscribed paralogue with One from Rp1-D, but amino-terminal portion another homologue, conferred specificity reduced level resistance.