N-Glycans: Phenotypic Homology and Structural Differences between Myocardial Cells and Induced Pluripotent Stem Cell-Derived Cardiomyocytes
作者： Takuji Kawamura , Shigeru Miyagawa , Satsuki Fukushima , Akira Yoshida , Noriyuki Kashiyama
DOI: 10.1371/JOURNAL.PONE.0111064
关键词:
摘要: Cell surface glycans vary widely, depending on cell properties. We hypothesized that glycan expression induced pluripotent stem cells (iPSCs) might change during cardiomyogenic differentiation toward the myocardial phenotype. N-glycans were isolated from iPSCs, iPSC-derived cardiomyocytes (iPSC-CM), and original C57BL/6 mouse myocardium (Heart). Their structures analyzed by a mapping technique based HPLC elution times MALDI-TOF/MS spectra. Sixty-eight different isolated; of 60 these identified. The quantity high-mannose type (immature) iPSCs decreased with differentiation, but did not reach low levels observed in heart. similar reduction neutral an increase fucosylated or sialyl N-glycans. Some structural differences detected between iPSC-CM Heart. No N-glycolyl neuraminic acid (NeuGc) iPSC-CM, whereas heart contained numerous NeuGc structures, corresponding to cytidine monophosphate-N-acetylneuraminic hydroxylase. Furthermore, several containing Galα1-6 Gal, rarely identified other cells, iPSC-CM. N-glycan murine changed phenotype leaving content Gal structures. Further studies will be warranted reveal meaning difference myocardium.
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