Simultaneous analysis method for GHB, ketamine, norketamine, phenobarbital, thiopental, zolpidem, zopiclone and phenytoin in urine, using C18 poroshell column.

摘要: Date-rape drugs have the potential to be used in drug-facilitated sexual assault, organ theft and property theft. Since they are colorless, tasteless odorless, victims can drink without noticing, when added beverages. These must detected time, before cleared up from biofluids. A simultaneous extraction determination method urine for GHB, ketamine, norketamine, phenobarbital, thiopental, zolpidem, zopiclone phenytoin (an anticonvulsant antiepileptic drug) with LC-MS/MS was developed first time analytically acceptable recoveries validated. 4 steps liquid-liquid applied, using only 1.000mL urine. new age commercial C18 poroshell column high efficiency analysis a fast isocratic elution as 5.5min. MS transition were introduced barbital. 222.7>179.8 effect of acetonitrile. Recoveries (%) between 80.98-99.27 all analytes, except GHB which 71.46. LOD LOQ values found ranges 0.59-49.50 9.20-80.80ngmL(-1) analytes (except GHB:3.44 6.00μgmL(-1)). HorRat calculated (between 0.25-1.21), revealed that inter-day interanalist precisions (RSD%≤14.54%) acceptable. The these 8 is challenging because difficulty arising different chemical properties some. procedure extract wide range polarity pKa, it increases window detection. Group representatives barbiturates, z-drugs, polar acidic (GHB) been successfully analyzed this study low detection limits. important point determining combined or single use crimes finding out reasons deaths related drugs.