Oxidation of intracellular glutathione after exposure of human red blood cells to hypochlorous acid.

摘要: Exposure of human red blood cells to low doses hypochlorous acid (HOCl) resulted in the loss intracellular GSH. Oxidation occurred less than 2 min after addition HOCl, and required approx. 2.5 mol HOCl per GSH lost. Loss preceded oxidation membrane thiols, formation chloramines haemoglobin oxidation. The susceptibility by was two-thirds that cell lysates. These results indicate can penetrate membrane, which provides little barrier protection for cytoplasmic components, may be a highly selective process. Virtually all lost converted into GSSG. If glucose added medium, most oxidized rapidly regenerated. At higher doses, recovery efficient. However, when as slow infusion rather single bolus, there increased at doses. This indicates metabolically active regeneration is rapid protect components from damage HOCl. HOCl-induced lysis only slightly delayed adding indicating lytic injury not ameliorated