Small interfering RNA targeting S100A4 sensitizes non-small-cell lung cancer cells (A549) to radiation treatment

摘要: OBJECTIVE This study aimed to investigate the impact of S100A4-small interfering RNA (S100A4-siRNA) on apoptosis and enhanced radiosensitivity in non-small-cell lung cancer (A549) cells. We also explored mechanisms radiosensitization identified a new target enhance gene therapy for cancer. METHODS interference is powerful tool silencing. In this study, we constructed an effective siRNA knock down S100A4. A549 cells were randomly divided into three groups: blank, negative control, S100A4-siRNA. To effect S100A4-siRNA, expression S100A4, E-cadherin, p53 proteins their messenger (mRNA) was detected by Western blot quantitative real-time polymerase chain reaction. Transwell chambers used assess cell invasion. Cell cycle analyzed flow cytometry. Radiosensitivity determined colony formation ability. RESULTS Our results demonstrate that S100A4-siRNA effectively silenced S100A4 gene. When against used, protein downregulated, whereas expressions E-cadherin upregulated. addition, clear reduction mRNA levels noted compared with blank control groups, increased. Transfection significantly reduced invasiveness silencing induced immediate G2/M arrest studies increased rates clonogenic assays, multitarget, single-hit model detect after knockdown. All parameters (D0, Dq, α, β) indicated downregulation Furthermore, upregulated expression, suggesting may promote proliferation, invasion, metastasis regulating other proteins. Therefore, siRNA-directed knockdown represent viable clinical CONCLUSION potentially enhances sensitivity human radiotherapy.