The purification, cloning, and expression of a novel luteinizing hormone-induced mitochondrial protein in MA-10 mouse Leydig tumor cells. Characterization of the steroidogenic acute regulatory protein (StAR).

摘要: The acute response of steroidogenic cells to trophic hormone stimulation is the mobilization cholesterol from cellular stores mitochondrial outer membrane and transfer this inner where first enzymatic step in steroidogenesis occurs. across membranes dependent upon de novo protein synthesis, regulated process. Although newly synthesized regulatory protein(s) have yet be identified, we previously proposed a candidate which identified MA-10 that luteinizing localized mitochondria. In present study, report isolation cDNA encodes hormone-induced protein. Analysis sequences reveals novel Importantly, demonstrate for time expression absence sufficient induce steroid production. We conclude required regulation propose call Steroidogenic Acute Regulatory (StAR).