Getting More Out of Less – A Quantitative Serological Screening Tool for Simultaneous Detection of Multiple Influenza A Hemagglutinin-Types in Chickens

摘要: Current avian influenza surveillance in poultry primarily targets subtypes of interest for the veterinary sector (H5, H7). However, as virological and serological evidence suggest, additional is important public health well industry. Therefore, we developed a protein microarray enabling simultaneous identification antibodies directed against different HA-types A viruses chickens. The assay successfully discriminated negative from experimentally naturally infected, seropositive Sensitivity specificity depended on cut-off level used but ranged 84.4% to 100% 100%, respectively, cut off ≥1∶40, showing minimal cross reactivity. As this testing platform also validated use humans, it constitutes tool that can be applied human-animal interface studies.