Development of an influenza virus protein microarray to measure the humoral response to influenza virus infection in mallards.

摘要: Avian influenza viruses pose a serious zoonotic threat, in part because current seasonal virus vaccines only offer strain-specific protection, instead of heterosubtypic or universal protection against infection. Understanding the humoral response to vaccination and natural infection broadest context possible is important developing defenses zoonosis. Protein microarrays are novel platform well suited assaying immune broadly efficiently. We developed an protein microarray (IVPM) that could be used assay sera from many species, including humans. Waterfowl such as mallard ducks reservoirs for A viruses, but their poorly understood. To establish this technology, we assayed mallards experimentally infected with two low-pathogenic common avian (H3N8 H4N5) reactivity hemagglutinin (HA) by IVPM. The IVPM results correlated established enzyme-linked immunosorbent assay, supporting validity serological research. Interestingly, successive H3N8 followed H4N5 induced antibodies were reactive group 2 hemagglutinins. also analyzed wild observed evidence those sera. With information, it may infer history species gain better understanding dynamics reservoir. This might ultimately lead interventions enhance our pandemic preparedness.