Blinded comparison of a direct immunofluorescent monoclonal antibody staining method and a Giemsa staining method for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens of patients infected with human immunodeficiency virus.

摘要: A new direct immunofluorescence monoclonal antibody (DFA) method (Genetic Systems, Inc., Seattle, Wash.) for identification of Pneumocystis carinii in induced sputum and bronchoalveolar lavage specimens was compared a blinded study with an established Giemsa stain method. We evaluated 148 consecutive clinical from 104 patients the following results. For 67 (64%) infected human immunodeficiency virus (HIV), 49 were initially negative by both DFA methods, none positive Giemsa, 6 12 sensitivity predictive value greater than 99%. six all methods on evaluation subsequently obtained specimens, suggesting specificity 99% false-positive rate less 1%. 37 whose HIV status or unknown, 35 2 Giemsa. The simple to perform required time scoring stained slides method, but care had be taken avoid readings due extraneous fluorescence. This indicates that represents advance as sensitive, simple, rapid way identify P. HIV-infected suggests this patient population.