Interspecies comparison of the glucuronidation processes in the man, monkey, pig, dog and rat.
作者： Pavel Anzenbacher , Petra Jancova , Michal Siller , Vlastimil Masek , Jaroslav Matal
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摘要: Abstract OBJECTIVES : The study of interspecies differences in glucuronidation processes the man, monkey, pig, dog and rat using liver microsomal fraction. is focused on determination enzyme activity UGT1A6 (having also a toxicological importance) microsomes different species. METHODS For glucuronides formed, an HPLC method with UV detection LC-MS characterization was used. p-Nitrophenol 4-methylum-belliferon silybin were chosen as model substrates. RESULTS data presented this paper show overall similarity kinetic parameters p-nitrophenol 4-methylumbelliferon for pig monkey. pattern formed monkey samples are relatively close to man. CONCLUSIONS studies xenobiotics where role expected, use should be consid-ered. As optimal glucuronidation, both rhesus (Beagle) seem best models. To elucidate UGT forms involved metabolism silybin, experiments recombinant enzymes needed.
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