Development of a liposome immunosorbent assay for human interferon-y
作者： H.A.H. Rongen , H.M. van der Horst , G.W.K. Hugenholtz , A. Bult , W.P. van Bennekom
DOI: 10.1016/0003-2670(93)E0592-U
关键词:
摘要: Abstract A novel and sensitive liposome immunosorbent assay (LISA) for IFN- y is described. Biotinylated liposomes with encapsulated carboxyfluorescien comprise a universal label immunoassays. Stability of the proven to be adequate long-term storage, without loss marker. Sensitivity LISA 6×10 −1 M comparable best colorimetric immunoassays , linear detection range (6−2400×10 −12 M) exceeds range. Analysis biological samples showed no interference on from potential disturbing factors in either plasma or blood cell culture supernatants.
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