Preparation of Antibody-Coupled Liposomes Containing Horseradish Peroxidase as a Marker Molecule
作者： Takahiro SUITA , Tamio KAMIDATE
关键词: Covalent bond 、 Luminol 、 Chemiluminescence 、 Synthetic membrane 、 Horseradish peroxidase 、 Chemistry 、 Peroxidase 、 Liposome 、 Chromatography 、 Conjugated system
摘要: Horseradish peroxidase (HRP) was encapsulated in liposomes prepared by an extrusion technique. The were coupled covalently to anti-rabbit IgG using N-hydroxysuccinimide ester palmitic acid as a component of liposomes. number HRP molecules per liposome about 800. A large portion inside the for one week at 4°C. catalytic activity measured luminol chemiluminescence (CL) method and found be almost constant during storage. CL intensity antibody detection antibody-coupled 125-times greater than that conjugated directly antibody.
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