Density gradient separation of carborane-containing liposome from low density lipoprotein and detection by inductively coupled plasma spectrometry.

摘要: Inductively coupled plasma atomic emission spectroscopy (ICP-AES) was used for analyzing the new cholesterol-based compounds (BCH, BCH-Da, BCH-Db and BCH-Dc) in liposomal formulations. Not only boron but also phospholipid compositions of liposome formulation were quantitatively analyzed. Reasonable limit detection (0.5 µg/ml) phosphorous (0.09 µg/ml), respectively, observed. ICP-MS utilized BCH a brain distribution study. The analysis by is at least three orders magnitude lower than that ICP-AES (1 ng B/ml). method linear range 500-1 B/ml linearity correlation coefficient 1. In addition, an ultracentrifugation developed to separate liposomes from low-density lipoprotein (LDL). Factors such as density gradient size adjusted optimize separation it observed conjunction time, speed gradient, had impact on using centrifugation method. These findings show importance analytical element-based encapsulated vesicles.