Analysis of exosome purification methods using a model liposome system and tunable-resistive pulse sensing.

摘要: Exosomes are vesicles which have garnered interest due to their diagnostic and therapeutic potential. Isolation of pure yields exosomes from complex biological fluids whilst preserving physical characteristics is critical for downstream applications. In this study, we use 100 nm-liposomes 1,2-dioleoyl-sn-glycero-3-phosphocholine (DOPC) cholesterol as a model system assess the effect exosome isolation protocols on vesicle recovery size distribution using single-particle analysis method. We demonstrate that liposome ζ-potential comparable extracted exosomes, making them an ideal comparison studies. Four different purification were evaluated, with liposomes robustly isolated by three them. Recovered varied was unaltered during processing, suggesting these do not induce particle aggregation. This leads us conclude profile stably maintained processing purification, reports detailing how derived tumour cells differ in those normal reporting real phenomenon. However, hypothesize larger particles present most purified samples represent co-purified contaminating non-exosome debris. These techniques therefore likely nonspecific may co-isolate material similar properties.