Determination of Peroxidase Encapsulated in Liposomes Using Homogentisic Acid γ-Lactone Chemiluminescence
作者： Tamio KAMIDATE , Nobuhito KIKUCHI , Akihiko ISHIDA , Hirofumi TANI
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摘要: Homogentisic acid γ-lactone (HAL) chemiluminescence (CL) was applied to the determination of horseradish peroxidase (HRP) encapsulated in liposomes. HRP detected after lysis HRP-trapped liposomes with Triton X-100. CL response rate, detection limit and linear range calibration curve for HAL were compared those p-iodophenol (p-IP)-enhanced luminol CL. Maximal light emission appeared more rapidly that p-IP enhanced CL, thus resulting remarkable reduction measurement time. The same as p-IP-enhanced improved by a factor 50 From these results, it found superior
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