Rapid electrophoretic microzone membrane technique for Regan isoenzyme (placental type alkaline phosphatase) using a fluorogenic substrate

摘要: Abstract Conditions are described for adapting Beckman Microzone cell electrophoresis to the detection and quantitation of Regan isoenzyme alkaline phosphatase. Successful use is made naphthol ASMX phosphate, a fluorogenic substrate, with result that minute (1 λ) quantities serum all necessary. In practice, specimens heat-inactivated without antibody placental phosphatase placed side-by-side on membrane electrophoresed. A positive identification if isozyme band in antigen-antibody position fails travel as far antigen position. This technique superior many respects starch gel previously employed.