Establishment and characterization of six human lung cancer cell lines: EGFR, p53 gene mutations and expressions of drug sensitivity genes

摘要: Six human lung cancer cell lines (SNU-371, SNU-963, SNU-1327, SNU-1330, SNU-2292 and SNU-2315) were newly established through primary cultures. These derived from a pulmonary blastoma, small cancer, three adenocarcinomas squamous carcinoma of the six Korean patients. The histopathology tumors their in vitro growth characteristics described. DNA fingerprinting analysis genetic alterations p53, β-catenin, TGFβRII, K-ras EGFR genes conducted. mRNA expressions levels E-cadherin, COX-2, MDR1, MXR, CGA, synatophysin TTF1 investigated sensitivity to anticancer drugs was screened. Five grew as adherent cells one line floating aggregates. All free mycoplasma or bacteria proven unique by analysis. A significant polymorphism at codon 72 (Arg Pro) p53 gene found (SNU-1327) mutation 176 SNU-2292. No mutations K-ras, β-catenin TGF-βRII observed. E-cadherin not expressed SNU-371 COX-2 overexpressed SNU-2315 lines. MDR1 MXR SNU-1327 line. Interestingly, blastoma which displayed cross resistance for several drugs. Neuroendocrine markers, chromogranin synaptophysin, line, SNU-963 thyroid transcription factor-1 also over this Two (p.Glu746_Ser752delinsVal p.Glu746_Ala750del) exon 19 SNU-1330 lines, respectively. well-characterized may be useful tools investigations biological cancers, particularly related EGFR.