Growth hormone promotes proliferation of adult neurosphere cultures.
作者： S. McLenachan , M.-G. Lum , M.J. Waters , A.M. Turnley
DOI: 10.1016/J.GHIR.2008.09.003
关键词:
摘要: Abstract Objectives Growth hormone (GH) and its receptor (GHR) are widely expressed in the CNS. During development, GH signaling regulates both proliferation of neural progenitor cells as well their differentiation into neurons glia. Here we have examined effect on adult subventricular zone derived cultured neurospheres. Design was added to wild-type (WT) neurosphere cultures growth measured using MTT cell assay. To examine influence endogenous production progenitors, neurospheres from knockout (GHRKO) mice were by measuring sizes Ki67 TUNEL immunoreactivity. In addition, curves compared following long term culture. Finally, WT vs. GHRKO immunocytochemistry for βIII-tubulin GFAP. Results While alone insufficient support formation, it enhanced 20% presence epidermal factor fibroblast factor-2. Compared wildtype neurospheres, smaller, contained fewer proliferating exhibited reduced self-renewal Addition increased STAT5 phosphorylation levels cells. Upon differentiation, showed accelerated neurogenesis, although over time similar numbers positive generated genotypes. Conclusions functions an autocrine mitogen promotes cultures. through GHR appeared delay neuronal
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