Amide cross-linking: an alternative to glutaraldehyde fixation.

摘要: Background and aims of the study A new fixation method for bioprosthetic tissues is being developed, which does not utilize standard glutaraldehyde treatment. This method, referred to as Ultifix, uses a coupler coupling enhancer with or without one more agents. It fixes tissue by linking amine carboxyl moieties through amide bonds either directly, indirectly when agents form bridges. The thus formed are stable than Schiff-base glutaraldehyde. All compounds used during process their by-products water-soluble, easily removed washing. In addition, toxic, opposed glutaraldehyde, induces toxic reactions after implantation. tests described in manuscript were specifically aimed at evaluating cross-linking efficacy on heart valve tissues, well resistance calcification rat model. Methods Porcine aortic roots porcine pericardium fixed using 1,6-hexane diamine (DIA) suberic acid (SUA) presence 1-ethyl-3(-3 dimethyl aminopropyl) carbodiimide hydrochloride (EDC) N-hydroxysulfosuccinimide (sulfo-NHS). then evaluated thermal denaturation, enzymatic digestion, implanted subdermally rats two, four, eight 16 weeks. Results results demonstrate that cusps wall roots, pericardium, stabilized cross-linked Ultifix they method. root but root, calcify minimally significantly less up weeks three week old control material Conclusion valves may be good alternative fixation, increased durability effects.