2-arachidonoylglycerol, an endogenous cannabinoid receptor ligand, induces rapid actin polymerization in HL-60 cells differentiated into macrophage-like cells.

摘要: Δ9-Tetrahydrocannabinol, a major psychoactive constituent of marijuana, interacts with specific receptors, i.e. the cannabinoid thereby eliciting variety pharmacological responses. To date, two types receptors have been identified: CB1 receptor, which is abundantly expressed in nervous system, and CB2 predominantly immune system. Previously, we investigated detail structure–activity relationship various receptor ligands found that 2-AG (2-arachidonoylglycerol) most efficacious agonist. We proposed true natural ligand for both receptors. Despite potential physiological importance 2-AG, not much information available concerning its biological activities towards mammalian tissues cells. In present study, examined effect on morphology as well actin filament system differentiated HL-60 cells, express receptor. induces rapid morphological changes such extension pseudopods. also it provokes polymerization these Actin induced by was abolished when cells were treated SR144528, antagonist, pertussis toxin, suggesting response mediated Gi/o. A phosphoinositide 3-kinase, Rho family small G-proteins tyrosine kinase suggested to be involved. Reorganization known indispensable cellular events; possible plays physiologically essential roles inflammatory immune-competent inducing rearrangement.