Characterization of a T cell line specific to an anti-Id antibody related to the carbohydrate antigen, sialyl SSEA-1, and the immundominant T cell antigenic site of the antibody.

摘要: The stage-specific embryonic Ag-1 (SSEA-1) is a carbohydrate Ag and regarded as an onco-developmental Ag. Sialyl SSEA-1 Ag, the sialylated form of SSEA-1, frequently expressed in human cancer cells well murine cells. A mAb, FH-6, was shown to specifically recognize We have generated five anti-Id Abs directed paratope-related idiotopes FH-6 Ab. One these Abs, Id-F2, increased survival host mice that were inoculated with Meth-A expressing sialyl To clarify exact mechanism underlying antitumor effect Ab, we established T cell line recognized Id-F2 association MHC class II molecules. CD4+V beta 8+, produced IL-2, exhibiting helper activity for B VH CDR2 region amino acid sequences turned out be strongly immunogenic When immune complexes, consisting formed at cell-surface, showed strong proliferative response. possible roles played by such subsets anti-tumor are discussed.