Identification and characterization of acetyltransferase-type toxin-antitoxin locus in Klebsiella pneumoniae
作者： Hongliang Qian , Qingqing Yao , Cui Tai , Zixin Deng , Jianhua Gan
DOI: 10.1111/MMI.13934
关键词:
摘要: A type II toxin-antitoxin (TA) system, in which the toxin contains a Gcn5-related N-acetyltransferase (GNAT) domain, has been characterized recently. GNAT acetylates aminoacyl-tRNA and blocks protein translation. It is abolished by cognate antitoxin that ribbon-helix-helix (RHH) domain. Here, we present an experimental demonstration of interaction GNAT-RHH complex with TA promoter DNA. First, locus kacAT was found Klebsiella pneumoniae HS11286, strain resistant to multiple antibiotics. Overexpression KacT halted cell growth resulted persister formation. The crystal structure also indicated typical acetyltransferase toxin. Co-expression KacA neutralized toxicity. Expression bicistronic up-regulated during antibiotic stress. Finally, formed heterohexamer interacted DNA, resulting negative autoregulation transcription. N-terminus region accounted for specific binding palindromic sequence on operator whereas its C-terminus essential inactivation These results provide important insight into regulation family system.
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