Early-life antibiotics attenuate regulatory T cell generation and increase the severity of murine house dust mite-induced asthma

摘要: Early-life exposure to antibiotics (ABX) has been linked increases in asthma severity and prevalence both children laboratory animals. We explored the immunologic mechanisms behind this association using a mouse model of house dust mite (HDM)-induced early-life ABX exposure. Mice were exposed three short courses following weaning experimental was thereafter induced. Airway cell counts differentials; serum immunoglobulin E (IgE); pulmonary function; lung histopathology; regulatory T cells (Tregs); fecal microbiome characterized induction asthma. Asthma increased mice ABX, including: airway eosinophilia, hyper-reactivity, HDM-specific IgE, histopathology. treatment led sharp reduction diversity, including loss pro-regulatory organisms such as Lachnospira. Pulmonary Tregs reduced with treatment, directly proportional diminished diversity. Intermittent early life worsened Tregs; latter change correlated decreased These data may suggest targets for or probiotic therapy counteract harmful effects childhood ABX.