Characterizing Protein‐Protein Interactions by Sedimentation Velocity Analytical Ultracentrifugation
作者： Patrick H. Brown , Andrea Balbo , Peter Schuck
DOI: 10.1002/0471142735.IM1815S81
关键词:
摘要: This unit introduces the basic principles and practice of sedimentation velocity analytical ultracentrifugation for study reversible protein interactions, such as characterization self-association, heterogeneous association, multi-protein complexes, binding stoichiometry, determination association constants. The tools described include coefficient molar mass distributions, multi-signal Gilbert-Jenkins theory, different forms isotherms, global Lamm equation modeling. Concepts experimental design are discussed, a detailed step-by-step protocol guiding reader through experiment data analysis is available an Internet resource. Curr. Protoc. Immunol. 81:18.15.1-18.15.39. © 2008 by John Wiley & Sons, Inc. Keywords: sedimentation equilibrium; sedimentation velocity; chemical equilibria; reversible interactions; multi-protein complex; analytical ultracentrifugation; size-distribution; Gilbert-Jenkins theory; Lamm equation; Bayesian
sci-hub.se 参考文章(102)
Peter Schuck, Sedimentation Velocity in the Study of Reversible Multiprotein Complexes Springer, Boston, MA. pp. 469- 518 ,(2007) , 10.1007/978-0-387-35966-3_16
D. I. Svergun, P. Vachette, Structure Analysis of Macromolecular Complexes by Solution Small-Angle Scattering Springer, Boston, MA. pp. 317- 365 ,(2007) , 10.1007/978-0-387-35966-3_11
Arthur Jennison Rowe, S. E. Harding, J. C. Horton, Analytical ultracentrifugation in biochemistry and polymer science Royal Society of Chemistry. ,(1992)
Lilo M. Gilbert, Geoffrey A. Gilbert, [9] Molecular transport of reversibly reacting systems: Asymptotic boundary profiles in sedimentation, electrophoresis, and chromatography Methods in Enzymology. ,vol. 48, pp. 195- 212 ,(1978) , 10.1016/S0076-6879(78)48011-5
William E. Werner, H.K. Schachman, Analysis of the ligand-promoted global conformational change in aspartate transcarbamoylase: Evidence for a two-state transition from boundary spreading in sedimentation velocity experiments☆ Journal of Molecular Biology. ,vol. 206, pp. 221- 230 ,(1989) , 10.1016/0022-2836(89)90535-4
William F Harrington, Gerson Kegeles, Pressure effects in ultracentrifugation of interacting systems. Methods in Enzymology. ,vol. 27, pp. 306- 345 ,(1973) , 10.1016/S0076-6879(73)27016-7
C. Urbanke, B. Ziegler, K. Stieglitz, Complete evaluation of sedimentation velocity experiments in the analytical ultracentrifuge Fresenius Journal of Analytical Chemistry. ,vol. 301, pp. 139- 140 ,(1980) , 10.1007/BF00467783
Stephen W. Provencher, Inverse problems in polymer characterization: Direct analysis of polydispersity with photon correlation spectroscopy Macromolecular Chemistry and Physics. ,vol. 180, pp. 201- 209 ,(1979) , 10.1002/MACP.1979.021800119
Julie Dam, Peter Schuck, Calculating sedimentation coefficient distributions by direct modeling of sedimentation velocity concentration profiles. Methods in Enzymology. ,vol. 384, pp. 185- 212 ,(2004) , 10.1016/S0076-6879(04)84012-6
J.M. Marcum, G.G. Borisy, Sedimentation velocity analyses of the effect of hydrostatic pressure on the 30 S microtubule protein oligomer. Journal of Biological Chemistry. ,vol. 253, pp. 2852- 2857 ,(1978) , 10.1016/S0021-9258(17)40900-8