POU2F1 induces the immune escape in lung cancer by up-regulating PD-L1.
作者： Tianyi Wang , Fei Li , Yinpeng Huang
DOI:
关键词:
摘要: Purpose The aim was to research the POU2F1 related genes and mechanism during progress of immune escape lung cancer. Methods Lung cancer cell lines (H1993, HCC827, A549, H2228, H3122 H1975) Human normal epithelial line (BEAS-2B) were involved in this study. Overexpression or knockdown processed cells. POU2F1, PD-L1 CRK expression cells detected by WB RT-PCR. Flow cytometry immunofluorescence used detect on surface. Luciferase reporter promoter activity CRK. C57BL/6 mice models with knocked down constructed. After tumor formation, anti-PD-1 administered suppressing ability. IHC assay showed number intratumoral CD3+, CD8+, GranzB+ T Results positively correlated lines. promoted level transcription activated CRK, further PD-L1. Knockdown efficacy Anti-PD-1. In addition, growth ability decreased after down. Cytotoxic effector cytokines levels, suppressive chemokines interleukin increased, while IL17a when Conclusion activates promotes PD-L1, finally improves
参考文章(37)
Song-Hui Xu, Jin-Zhou Huang, Man-Li Xu, Guangchuang Yu, Xing-Feng Yin, De Chen, Guang-Rong Yan, ACK1 promotes gastric cancer epithelial–mesenchymal transition and metastasis through AKT–POU2F1–ECD signalling The Journal of Pathology. ,vol. 236, pp. 175- 185 ,(2015) , 10.1002/PATH.4515
Mariëlle W.M. Muskens, Adriënne P.A. Vissers, Joseph N.M. Mol, Jan M. Kooter, Role of inverted DNA repeats in transcriptional and post-transcriptional gene silencing. Plant Molecular Biology. ,vol. 43, pp. 243- 260 ,(2000) , 10.1023/A:1006491613768
Juan Cong Dong, Hui Gao, Si Yao Zuo, Hai Qin Zhang, Gang Zhao, Shi Long Sun, Hai Ling Han, Lin Lin Jin, Li Hong Shao, Wei Wei, Shun Zi Jin, Neuropilin 1 expression correlates with the Radio-resistance of human non-small-cell lung cancer cells Journal of Cellular and Molecular Medicine. ,vol. 19, pp. 2286- 2295 ,(2015) , 10.1111/JCMM.12623
Jerzy Paszkowski, Steven A Whitham, Gene silencing and DNA methylation processes. Current Opinion in Plant Biology. ,vol. 4, pp. 123- 129 ,(2001) , 10.1016/S1369-5266(00)00147-3
Cheng Chen, Xue-Guang Zhang, Chuan-Yong Mu, Jian-An Huang, Ying Chen, High expression of PD-L1 in lung cancer may contribute to poor prognosis and tumor cells immune escape through suppressing tumor infiltrating dendritic cells maturation Medical Oncology. ,vol. 28, pp. 682- 688 ,(2011) , 10.1007/S12032-010-9515-2
Edward B Garon, Naiyer A Rizvi, Rina Hui, Natasha Leighl, Ani S Balmanoukian, Joseph Paul Eder, Amita Patnaik, Charu Aggarwal, Matthew Gubens, Leora Horn, Enric Carcereny, Myung-Ju Ahn, Enriqueta Felip, Jong-Seok Lee, Matthew D Hellmann, Omid Hamid, Jonathan W Goldman, Jean-Charles Soria, Marisa Dolled-Filhart, Ruth Z Rutledge, Jin Zhang, Jared K Lunceford, Reshma Rangwala, Gregory M Lubiniecki, Charlotte Roach, Kenneth Emancipator, Leena Gandhi, None, Pembrolizumab for the treatment of non-small cell lung cancer The New England Journal of Medicine. ,vol. 372, pp. 2018- 2028 ,(2015) , 10.1056/NEJMOA1501824
Na Huang, Jing Zhu, Dan Liu, Ya-Lun Li, Bo-Jiang Chen, Yan-Qi He, Kun Liu, Xian-Ming Mo, Wei-Min Li, Overexpression of Bcl-2–Associated Death Inhibits A549 Cell Growth In Vitro and In Vivo Cancer Biotherapy and Radiopharmaceuticals. ,vol. 27, pp. 164- 168 ,(2012) , 10.1089/CBR.2011.1018
Annie M. Brown, The pattern, sensitivity and precision of the response to insulin in random bred, inbred and hybrid strains of mice. Journal of Pharmacy and Pharmacology. ,vol. 13, pp. 670- 678 ,(2011) , 10.1111/J.2042-7158.1961.TB11890.X
Dongfang Liu, The adaptor protein Crk in immune response Immunology and Cell Biology. ,vol. 92, pp. 80- 89 ,(2014) , 10.1038/ICB.2013.64
Stephen B. Baylin, Joyce E. Ohm, Epigenetic gene silencing in cancer – a mechanism for early oncogenic pathway addiction? Nature Reviews Cancer. ,vol. 6, pp. 107- 116 ,(2006) , 10.1038/NRC1799