CD109 is a component of exosome secreted from cultured cells
作者： Hiroki Sakakura , Shinji Mii , Sumitaka Hagiwara , Takuya Kato , Noriyuki Yamamoto
DOI: 10.1016/J.BBRC.2015.12.063
关键词:
摘要: Exosomes are 50-100-nm-diameter membrane vesicles released from various types of cells. retain proteins, mRNAs and miRNAs, which can be transported to surrounding CD109 is a glycosylphosphatidylinositol-anchored glycoprotein, the cell surface culture medium in vitro. Recently, it was reported that secreted downregulates transforming growth factor-β signaling human keratinocytes. In this study, we revealed component exosome conditioned medium. FLAG-tagged (FLAG-CD109) HEK293 cells expressing FLAG-CD109 (293/FLAG-CD109) immunoprecipitated with anti-FLAG affinity gel, co-precipitated proteins were analyzed by mass spectrometry western blotting. Exosomal associated CD109. We presence fractions 293/FLAG-CD109. Moreover, localization demonstrated using immuno-electron microscopy. When used truncated CD109, does not contain C-terminal region, association exosomes detected These findings indicate an exosomal protein region required for its exosome.
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