CD109 mediates tumorigenicity and cancer aggressiveness via regulation of EGFR and STAT3 signalling in cervical squamous cell carcinoma.

摘要: CD109 was involved in the tumorigenesis and progression of various cancers via TGF-β1 signalling STAT3 activation. As is strongly expressed cervical squamous cell carcinoma, this study conducted to investigate its functional characteristics cancer. expression examined by immunohistochemistry (IHC) with tissue microarray. The effects were on migration, proliferation, spheroid formation soft-agar colony-formation assay. Meanwhile, cancer lines high chosen for using siRNA knockdown CRISPR/Cas9 knockout. IHC demonstrated an upregulation membrane carcinoma. CD109( + ) cells isolated flow-cytometric sorting displayed enhanced sphere-forming anchorage-independent growth ability. In contrast, silencing could reverse vitro vivo tumorigenic aggressive properties. Furthermore, induced EGFR-mediated phosphorylation known be responsible proliferation maintenance CSC phenotype. Abundant populations potentially contributed carcinogenesis aggressiveness, whereas those mediates tumorigenicity aggressiveness CD109/EGFR/STAT3 signalling.