An Lyt differentiated thymocyte subpopulation detected by flow microfluorometry

摘要: DATA on the T-cell antigens, Lyt 1, 2, 3 (refs 2), relating to functional subsets, suggest that some peripheral T cells express restricted phenotypes, is, 1+23− or 1−23+ (ref. 3). Evidence has been presented showing two major subpopulations of lymphocytes, 1+ and 23+ cells, are committed particular lines differentiation in immune response before encountering exogenous antigen lymphoid tissue4,5. It suggested representing help (Lyt 1+) cytotoxicity suppression 23+) arise periphery from 123+ cells6. Recent studies have concluded as stem mature thymus, thymic epithelium is important determining which H–2 specificities maturing able recognise self7. Previous data interpreted indicating antigens expressed each most if not all, mouse thymocytes; thymocytes 1–4, 8–10). However, here commitment phenotype, thus presumably relevant occurs cell migration thymus. Using flow microfluorometry (FMF) anti-Lyt antisera, we demonstrate a normal thymocyte subpopulation expressing 2 1+23−). This represents significant constant proportion (10%) markedly increased following vivo cortisone treatment. The also show 1 expression, rather than being decreased cortisone-resistant (CRT), would predicted previous data2,8, amounts CRT.