The exostosin family of glycosyltransferases: mRNA expression profiles and heparan sulphate structure in human breast carcinoma cell lines

摘要: Breast cancer remains a leading cause of cancer-related mortality in women. In recent years, regulation genes involved heparan sulphate (HS) biosynthesis have received increased interest as regulators breast cell adhesion and invasion. The exostosin (EXT) proteins are glycosyltransferases elongation HS, regulator intracellular signaling, cell-cell interactions, tissue morphogenesis. EXT family contains five members: EXT1, EXT2, three EXT-like (EXTL) EXTL1, EXTL2, EXTL3. While the expression levels these enzymes change tumor cells, little is known how this changes structure function HS. present study, we investigated gene profiles members, their glycosyltransferase activities HS estrogen receptor (ER), progesterone (PR) positive MCF7 ER, PR, human epidermal growth factor receptor-2 (HER2) negative MDA-MB-231 HCC38 epithelial carcinoma lines. for cells were very similar. both lines EXTL2 was found to be up-regulated whereas EXT2 down-regulated. Interestingly, despite having similar two synthesized chains significantly different lengths. Furthermore, exhibited markedly decreased 6-O-sulphated disaccharides. Although did not correlate with length chains, our results indicated specific differences enzyme fine characteristic carcinogenic properties cells.