Age Impacts Pulmonary Inflammation and Systemic Bone Response to Inhaled Organic Dust Exposure.

摘要: Agricultural workers have high rates of airway and skeletal health disease. Studies recently demonstrated that inhaled agricultural organic dust extract (ODE)-induced injury is associated with bone deterioration in an animal model. However, the effect age governing these responses to dusts unclear, but might be important future approaches. Young (7-9 wk) older (12-14,o) male C57BL/6 mice received intranasal (i.n.) inhalation exposure ODE from swine confinement facilities once or daily for 3 wk. Acute ODE-induced neutrophil influx cytokine chemokine (tumor necrosis factor [TNF]-α, interleukin [IL]-6, keratinocyte chemoattractant [CXCL1], macrophage inflammatory protein-2 [CXCL2]) production were reduced compared young mice. Repetitive treatment, however, increased lymphocyte recruitment alveolar compartment histopathologic changes Whole lung cell infiltrate analysis revealed young, not older, repetitively treated elevated CD4:CD8 response. inhalant resulted a 4-fold 1.5-fold rise blood neutrophils mice, respectively. Serum IL-6 CXCL1 levels i.n. exposed ODE, younger Although displayed measurements rodents decrease mineral density, volume, microarchitecture quality as determined by computed tomography (CT) analysis. Collectively, impacts systemic loss response suggesting altered enhanced immunologic counterparts.