Two step single primer mediated polymerase chain reaction. Application to cloning of putative mouse, β-galactoside α2,6-sialyltransferase cDNA
作者： Toshiro Hamamoto , Mikiko Kawasaki , Nobuyuki Kurosawa , Takashi Nakaoka , Young-Choon Lee
DOI: 10.1016/S0968-0896(00)82111-2
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摘要: Abstract Using the 2 step single primer mediated polymerase chain reaction(PCR), mouse β-galactoside (α2,6-sialyltransferase cDNA was cloned. Single PCR is a method to amplify particular DNA fragment beyond its known sequence region. It employs only one for reaction. Compared other methods an adjacent of fragment, this requires no enzymatic manipulation on template and applicable long fragment. First, short enzyme obtained from by usual using degenerate primers synthesized according relatively conserved region in rat human (α2,6-sialyltransferase. Four were based sequence, then performed obtain 5′ 3′ flanking sequences resulting 1.0 kb 1.3 fragments being amplified respectively. The integrity two confirmed additional joined which contained 1.2kb complete putative α2,6-sialyltransferase coding result showed that specificity consequently applicability amplifying remarkably improved successive 2nd
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sci-hub.se 参考文章(13)
J C Paulson, K J Colley, Glycosyltransferases. Structure, localization, and control of cell type-specific glycosylation. Journal of Biological Chemistry. ,vol. 264, pp. 17615- 17618 ,(1989) , 10.1016/S0021-9258(19)84610-0
D X Wen, E C Svensson, J C Paulson, Tissue-specific alternative splicing of the beta-galactoside alpha 2,6-sialyltransferase gene. Journal of Biological Chemistry. ,vol. 267, pp. 2512- 2518 ,(1992) , 10.1016/S0021-9258(18)45909-1
K.J. Colley, E.U. Lee, J.C. Paulson, The signal anchor and stem regions of the beta-galactoside alpha 2,6-sialyltransferase may each act to localize the enzyme to the Golgi apparatus. Journal of Biological Chemistry. ,vol. 267, pp. 7784- 7793 ,(1992) , 10.1016/S0021-9258(18)42583-5
J Weinstein, E U Lee, K McEntee, P H Lai, J C Paulson, Primary structure of beta-galactoside alpha 2,6-sialyltransferase. Conversion of membrane-bound enzyme to soluble forms by cleavage of the NH2-terminal signal anchor. Journal of Biological Chemistry. ,vol. 262, pp. 17735- 17743 ,(1987) , 10.1016/S0021-9258(18)45441-5
Tony Triglia, M.Gregory Peterson, David J. Kemp, A procedure for in vitro amplification of DNA segments that lie outside the boundaries of known sequences Nucleic Acids Research. ,vol. 16, pp. 8186- 8186 ,(1988) , 10.1093/NAR/16.16.8186
Ulrich Grundmann, Claudia Nerlich, Thomas Rein, Gerd Zettlmeissl, Complete cDNA sequence encoding human β-galactoside α-2, 6-sialyltransferase Nucleic Acids Research. ,vol. 18, pp. 667- 667 ,(1990) , 10.1093/NAR/18.3.667
O. Ohara, R. L. Dorit, W. Gilbert, One-sided polymerase chain reaction: the amplification of cDNA. Proceedings of the National Academy of Sciences of the United States of America. ,vol. 86, pp. 5673- 5677 ,(1989) , 10.1073/PNAS.86.15.5673
WEN-PIN WANG, RENE L. MYERS, ING-MING CHIU, Single primer-mediated polymerase chain reaction : application in cloning of two different 5'-untranslated sequences of acidic fibroblast growth factor mRNA DNA and Cell Biology. ,vol. 10, pp. 771- 777 ,(1991) , 10.1089/DNA.1991.10.771
E. Loh, J. Elliott, S Cwirla, L. Lanier, M. Davis, Polymerase chain reaction with single-sided specificity: analysis of T cell receptor delta chain. Science. ,vol. 243, pp. 217- 220 ,(1989) , 10.1126/SCIENCE.2463672
Christopher L. Parks, Long-Sheng Chang, Thomas Shenk, A polymerase chain reaction mediated by a single primer : cloning of genomic sequences adjacent to a serotonin receptor protein coding region Nucleic Acids Research. ,vol. 19, pp. 7155- 7160 ,(1991) , 10.1093/NAR/19.25.7155