Expression cloning of a GM3-specific alpha-2,8-sialyltransferase (GD3 synthase).
作者： K. Sasaki , K. Kurata , N. Kojima , N. Kurosawa , S. Ohta
DOI: 10.1016/S0021-9258(17)40773-3
关键词:
摘要: Abstract A cDNA encoding a GM3-specific alpha-2,8-sialyltransferase (GD3 synthase) was obtained from an expression library of human melanoma cell line WM266-4 by enrichment Namalwa KJM-1 cells highly expressing GD3 using anti-GD3 antibody and fluorescence-activated sorter. Selection B-cell transfected cDNAs in the presence monoclonal KM641 gave (pAMo-GD3) protein with type II transmembrane topology as found for mammalian glycosyltransferases. The following evidence confirms that encodes alpha-2,8-sialyltransferase, which specifically converts GM3 to GD3. (i) Transfection pAMo-GD3 into leads appearance synthase activity. (ii) Northern blot analysis revealed correlation between this gene several lines. (iii) putative COOH-terminal active domain cloned enzyme fused has been purified IgG-Sepharose beads shown possess GD3-synthesizing activity, excluding possibility transacting factor inducing synthase. deduced primary sequence also contains "sialyl motif" conserved among all sialyltransferases date. polymerase chain reaction reveals is located on chromosome 12.
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