Vaccine-Induced Carbohydrate-Specific Memory B Cells Reactivate During Rodent Malaria Infection.

摘要: A long-standing challenge in malaria is the limited understanding of B cell immunity, previously hampered by lack tools to phenotype rare antigen-specific cells. Our aim was develop a method for identifying carbohydrate-specific cells within lymphocyte populations and determine whether candidate vaccine generated functional memory (MBCs) that reactivated upon with Plasmodium (pRBCs). To this end, new flow cytometric probe validated used kinetics activation against glycosylphosphatidylinositol conjugated Keyhole Limpet Haemocyanin (GPI-KLH). Additionally, immunized C57BL/6 mice were rested (10 weeks) challenged pRBCs or GPI-KLH assess recall foreign antigen. We found GPI-specific detectable vaccinated mice, but not Plasmodium-infected mice. IgG1 MBCs both synthetic GPI-KLH, which resulted increased serum levels anti-GPI IgG approaches. Collectively our findings contribute immunity have important clinical implications inclusion carbohydrate conjugates vaccines.