Synapse preservation and decreased glial reactions following ventral root crush (VRC) and treatment with 4-hydroxy-tempo (TEMPOL).

摘要: Astrogliosis and microglial reactions are correlated with the formation of scar tissue synapse loss. 4-hydroxy-tempo (TEMPOL) is a reactive oxygen species scavenger proven neuroprotective efficacy in experimental models traumatic injury cerebral ischemia. TEMPOL has not, however, been applied following ventral root lesions, which particularly degeneration spinal motoneurons brachial plexus injuries. Thus, present study investigated effects on adjacent glial reactions, particular focus preservation excitatory inhibitory circuits. Adult female Sprague Dawley rats were subjected to crush (VRC) at lumbar intumescence. Animals divided into groups: (a) VRC-saline treatment; (b) VRC-TEMPOL treatment (12 mg/kg, n = 5), (c) (250 5). The cord located contralateral lesion was used as control. Fourteen days after lesioning, euthanized cords removed for motoneuron counting immunolabeling (GFAP Iba-1) markers (synaptophysin, VGLUT-1, GAD65). Although did not exert studied concentrations, modulation significant higher doses. synaptophysin staining preserved and, particular, VGLUT-1-positive inputs maintained, thereby indicating that proprioceptive glutamatergic without exacerbating rate degeneration. Consequently, its administration other efficient substances may significantly improve outcomes lesioning.