Route of inoculation influences Trypanosoma congolense and Trypanosoma brucei brucei virulence in Swiss white mice.

摘要: Experiments on infections caused by trypanosomes are widely performed in Swiss white mice through various inoculation routes. To better understand the effect of route trypanosome disease outcomes this model, we characterised virulence two isolates, Trypanosoma brucei KETRI 2710 and T. congolense 2765 mice. For each five routes parasite inoculation, namely intraperitoneal (IP), subcutaneous (SC), intramuscular (IM) intradermal (ID) intravenous (IV) were compared using groups (n = 6) mice, with mouse receiving 1x104 trypanosomes. We subsequently assessed impact indices that included pre-patent period (PP), parasitaemia levels, Packed Cell Volume (PCV), bodyweight changes survival time. Pre-patent for IP inoculated was a mean ± SE 3.8 0.2 6.5 0.0 T isolates respectively; PP other not significantly different(p> 0.05) irrespective species With ID routes, higher lower infected progression to peak showed no significant different between either trypanosome. The IM inoculations, IV b. induced fastest slowest progressions respectively. There differences rates reduction PCV time post infection which more pronounced sc ip injected No body weight survivorship observed inoculation. Inoculation therefore appears be critical determinant pathogenicity murine model African trypanosomiasis.