Recombinant Probes for Visualizing Endogenous Synaptic Proteins in Living Neurons

摘要: SUMMARY The ability to visualize endogenous proteins in living neurons provides a powerful means interrogate neuronal structure and function. Here we generate recombinant antibody-like proteins, termed Fibronectin intrabodies generated with mRNA display (FingRs), that bind PSD-95 Gephyrin high affinity that, when fused GFP, allow excitatory inhibitory synapses be visualized neurons. Design of the FingR incorporates transcriptional regulation system ties expression level target reduces background fluorescence. In dissociated brain slices, FingRs against did not affect patterns their or number strength synapses. Together, our data indicate can report localization amount synaptic thus may used study changes vivo.