[18O]‐Peroxides: Synthesis and Biological Applications

摘要: The peroxidation reactions of the main cellular targets including nucleic acids, proteins and unsaturated lipids have received major attention during last two decades. This was particularly case for hydroperoxides generated by 1O2, one reactive oxygen species that usually oxidizes biomolecules in a more specific way than either •OH or one-electron oxidants. Most endoperoxides produced 1O2 been shown to be unstable therefore their identification is often difficult. use [18O]-labeled released from thermolabile association with HPLC-ESI-MS/MS analysis provides highly suitable gain relevant mechanistic insights into formation decomposition pathways initially peroxidic compounds. possibility cell penetration afforded naphthalene has also exploited investigating reaction DNA accrediting mechanism oxidation guanine nucleus providing an elegant approach assessing validity isolation methods used measurement 8-oxo-7,8-dihydroguanine, ubiquitous product. Further developments are expected both studies applications cells using generators. Keywords: [18O]-Peroxides; hydroperoxides; [18O]-labeled singlet molecular oxygen; mass spectrometry; [18O]-labeled oxygen; naphthalene endoperoxides; DNA; protein; cardiolipin; lipid