Stimulation by G protein βγ subunits of phospholipase C β isoforms in human platelets

摘要: Different phospholipase C (PLC) isoforms were located in human platelet cytosol and membranes. PLCg2 PLCb3b mainly the PLCb2 PLCb3a both membranes by using specific antibodies against PLC isozymes (Banno Y, Nakashima S, Ohzawa M, Nozawa Y. J Biol Chem 1996; 271: 14989-94). Three fractions activated G protein bg subunits purified from membrane fractions. Two identified as PLCb3a, one was PLCb3b. These PLCb of brain proteins order > PLCb2. Western blot analysis g with various standard revealed that major component subunit Gi2 Gq g5, g7 a minor component. Studies subtypes subunits, bg2, bg3, bg7 bovine brain, bg5 lung, or bg12 spleen, failed to show differences their ability stimulate isolated isoforms. results suggest have similar efficacy regulation effectors platelets.