The influence of relative humidity on vitrification, growth and morphology of Gypsophila paniculata L.

摘要: A vitrification scoring system was devised that comprised a visual assessment of in vitro followed by transplanting plantlets ex and recording plantlet survival rates. This proved to be simple method predicting demonstrated severity is more important than acclimitization procedures promoting vitro. Growth normal liquid medium an environment where are able transpire made it clear lack transpiration as opposed high water availability the primary cause for induction vitrified growth. Finally, small decreases relative humidity were found sufficient increase extent greatly reduced increased. Small differences also influenced morphology with grown at higher being larger consisting shoots lower humidity. It suggested may manipulated produce particular should measured tissue culture experiments included materials methods sections papers ensure correct interpretation results.